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Experimental Observations and Numerical Modeling of Coupled Microbial and Transport Processes in Variably Saturated Sand

^a Pacific Northwest National Lab., P.O. Box 999/MSIN K9-36, Richland, WA 99352
^b Dep. of Crop and Soil Sciences, Oregon State Univ., Corvallis, OR 97331
^c CH2M Hill, 2300 NW Walnut Blvd., Corvallis, OR 97330
^d Dep. of Microbiology, Nash Hall, Oregon State Univ., Corvallis, OR 97331
^e Dep. of Bioengineering, Oregon State Univ., Corvallis, OR 97331

View larger version (46K): [in a new window]   Fig. 1. Setup for column experiments. Components include: carboys containing aqueous influent solutions of minimal mineral salts and glucose (1); peristaltic pump (2), drip emitters (3); housing containing ultraviolet lights (4); segmented, 55-cm-long acrylic columns with ring tensiometers, pressure transducers, and TDR probes (5); syringes for dissolved oxygen sampling (6); carboy for collection of waste effluent (7); Tektronix Model 11801 digital sampling oscilloscope (8); and computer with data acquisition board (8).

View larger version (19K): [in a new window]   Fig. 2. Hydraulic properties of clean sand and attached biomass phase that were combined using the composite media model described by Rockhold et al. (2002).

View larger version (19K): [in a new window]   Fig. 3. Capillary pressure scaling function.

View larger version (38K): [in a new window]   Fig. 4. Observed and simulated effluent glucose, dissolved oxygen, and biomass concentrations, and final, sand-associated (attached) biomass distributions.

View larger version (45K): [in a new window]   Fig. 5. Observed and simulated time histories of water content and pressure heads at selected depths.

View larger version (37K): [in a new window]   Fig. 6. Water retention data with Brooks–Corey (1964) model fit for clean sand, and data measured from tensiometers and TDR probes during the column experiment.